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Calvert R et al. Genetic abnormalities during transition from Helicobacter-pylori-associated gastritis to low-grade MALToma. Lancet 1995; 345 8941 ; : 26-7. Abstract Datta YH et al. Rituximab-induced remission of a gastric MALT lymphoma. Leuk Lymphoma 2004; 45 6 ; : 1297-9. Abstract DeJong D et al. Histological grading with clinical relevance in gastric mucosa-associated lymphoid tissue MALT ; lymphoma. Recent Results Cancer Res 2000; 156: 27-32. Abstract Du MQ, Isaacson PG. Gastric MALT lymphomas: From aetiology to treatment. Lancet Oncol 2002; 3 2 ; : 97-104. Abstract Fujimori K et al. Effect of Helicobacter pylori eradication on ongoing mutation of immunoglobulin genes in gastric MALT lymphoma. Br J Cancer 2005; [Epub ahead of print]. Abstract Inagaki H et al. Gastric MALT lymphomas are divided into three groups based on responsiveness to Helicobacter pylori eradication and detection of API2-MALT1 fusion. J Surg Pathol 2004; 28 12 ; : 1560-7. Abstract Isaacson PG, Du MQ. Gastrointestinal lymphoma: Where morphology meets molecular biology. J Pathol 2005; 205 2 ; : 255-74. Abstract Jaskiewicz K, Kobierska G. Lymphoid aggregates in gastric biopsies: Relationship to other mucosal lesions. Arch Immunol Ther Exp Warz ; 2000; 48 3 ; : 201-4. Abstract Marshall BJ, Windsor HM. The relation of Helicobacter pylori to gastric adenocarcinoma and lymphoma: Pathophysiology, epidemiology, screening, clinical presentation, treatment, and prevention. Med Clin North 2005; 89 2 ; : 313-44. No abstract available Martinelli G et al. Clinical activity of rituximab in gastric marginal zone non-Hodgkin's lymphoma resistant to or not eligible for anti-Helicobacter pylori therapy. J Clin Oncol 2005; [Epub ahead of print]. Abstract Morgner A et al. Helicobacter-induced expression of Bcl-X L ; in B lymphocytes in the mouse model: A possible step in the development of gastric mucosa-associated lymphoid tissue MALT ; lymphoma. Int J Cancer 2001; 92 5 ; : 634-40. Abstract O'Rourke JL et al. Gastric B-cell mucosa-associated lymphoid tissue MALT ; lymphoma in an animal model of `Helicopacter heilmannii' infection. J Pathol 2004; 203 4 ; : 896-903. Abstract Steinbach G et al. Antibiotic treatment of gastric lymphoma of mucosa-associated lymphoid tissue: An uncontrolled trial. Ann Intern Med 1999; 131 2 ; : 88-95. Abstract Sugiyama T, Asaka M. Helicobacter pylori infection and gastric cancer. Med Electron Micros 2004; 37 3 ; : 149-57. Abstract Taji S et al. Trisomy 3 may predict a poor response of gastric MALT lymphoma to Helicobacter pylori eradication therapy. World J Gastroenterol 2005; 11 1 ; : 89-93. Abstract Thomas DA et al. Outcome with the hyper-CVAD regimens in lymphoblastice lymphoma. Bolld 2004; 104 6 ; : 1624-30. Abstract Tsang RW et al. Localized mucosa-associated lymphoid tissue lymphoma treated with radiation therapy has excellent clinical outcome. J Clin Oncol 2003; 21 22 ; : 4157-64. Abstract Tsang RW et al. Stage I and II MALT lymphoma: Results of treatment with radiotherapy. Int J Radiat Oncol Biol Phys 2001; 50 5 ; : 1258-64. Abstract Venizelos I et al. Primary gastric Hodgkin's lymphoma: A case report and review of the literature. Leuk Lymphoma 2005; 46 1 ; : 147-50. Abstract.

1100 cpm, compared to 20 cpm for unalkylated enzyme i.e. enzyme treated with ["CIBrNAP in the presence of excess DTT to prevent chemical modification of the protein ; . To determine the half-life of BrNAP in aqueous solution, ["C] BrNAP was added at a final concentration of 10 to potassium phosphate buffer 100 mM, pH 7.4 ; at room temperature. Aliquots 8 x 0.5 ml ; were removed over a 6-h period and extracted with an equal volume of ethyl acetate. An aliquot 50 pl ; of the ethyl acetate extract which removed 97% of the radioactivity from the aqueous phase a t each time point ; was applied to the prebinder region of Whatman LK5DF silica TLC plates. To facilitate the localization of unchanged [14C]BrNAP under uv light, unlabeled BrNAP was applied to the TLC plates, which were then developed in benzene. The region of the plate containing BrNAP was scraped directly into a scintillation vial, and radioactivity was determined. To determine the apparent solubility of BrNAP in aqueous solution, 10 pl of 85 [I4C]BrNAP dissolved in acetonitrile ; was added M to 0.4 ml of potassium phosphate buffer 100 mM, pH 7.4 ; at room temperature. Insoluble ["CIBrNAP was removed by centrifugation followedby filtration through Whatman No. 1 filter paper. From measurements of the radioactivity in the clear filtrate, the apparent solubility of BrNAP was estimated to be -600 p ~ . Release of 4-Thiopyridone from PDS-treated Cytochrome P-45OcCytochrome P-45Oc that had been treated with sodium sulfite, DTT, and exhaustively dialyzed was treated with 2 molof PDS mol of cytochrome for 30 min at room temperature. The sample was then dialyzed overnight against 2X 2 liters 100 m potassium phosphate of M buffer pH 7.4 ; containing 20% glycerol.PDS-treated cytochrome P450c 5 nmol ; , with or without reconstitution with 16 nmol of NADPH-cytochrome P-450 reductase and 240nmol of dilauroylphosphatidylcholine, was incubated a t 4 for 5 min in atotal volume of 5 ml containing 1m NADPH, 100 m potassium phosphate pH M M 7.4 ; and 3 m MgC12. M Following the incubation, 1.64 mlof acetone and 0.5 mlof l-chloro2, 4-dinitrobenzene 0.1 M in acetone ; were added to each 5-ml incubation mixture, and theresultant solutions were incubated for 1 h at "C. The mixtures were cooled and acidified to pH -2 with 1 ml of hydrochloric acid and washed 3 times with 2-ml portions of hexane to remove most of the unreacted l-chloro-2, 4-dinitrobenzene. The combined hexane solutions were extracted with 1-ml portionsof 0.01 M hydrochloric acid, whichwere combined with the aqueous phase from the hexane extraction. This acidic aqueous solution, which contained the trapped product, 1- 4-pyridinethi0 ; -2, 4-dinitrobezene, in the protonated form was adjusted to pH -9-10 with 1.15 ml of 1 sodium hydroxide and extracted 3 times with 2-ml portions of ethyl acetate. The ethyl acetatesolution was dried with sodium sulfate and evaporated to dryness under a stream of nitrogen. The residue was dissolved in 0.2 ml of methanol and 0.2 ml of water, and 50- or 100pl samples were analyzed by HPLC on a Perkin-Elmer C18 column 3 p ; eluted with methanol, 0.05 M Tris-acetate buffer pH 7 ; , 45: 55, at a flow rate of 2 ml min. Under these conditions the trappedproduct from 4-thiopyridone, l- pyridinethio ; -2, 4-dintrobenzene ; , eluted a t is 3.3 min. 4-Thiopyridone was quantified by integration of this peak area, detected a t 330 nm, and comparison with astandard line generated by injection of dilutions containing 0.5-2.0 nmol of product ; of a mixture of authentic 4-thiopyridone 92 p ~ and 7 m 1 ; chloro-2, 4-dinitrobenzene that had been allowed to react under the conditions described above. Reproducibility of injections was ensured by the use of aPerkin-Elmer ISS-100 autosampler. In separate experiments, the trapped product was shown to be extracted, quantitatively by the procedure described, and Emulgen 911, dilauroylphosphatidylcholine and NADPH, at the concentrations used in the enzyme studies, did not interfere with its detection or quantitation. The identity of the product derived from the PDS-treated enzyme , with that derived from authentic 4-thiopyridone was verified by measurement of on-line UV spectra of the chromatographic peaks with an LKB model 2140diode-array detector ; and by measurement of the mass spectra of the eluted compounds after extraction into ethyl acetate CI, NH3 m z of base peak 278 M 1 ; . Catalytic Actiuity-The hydroxylations of aniline, benzo[a]pyrene, [4, 6-3H]zoxazolamine, [4-"C]testosterone, and [1, 2-3H]5P-androstane-3~~, 17&disulfate, the 0-dealkylation of 7-ethoxycoumarin, and HCl were dethe N-demethylation of [N-methyl-'4C]benzphetamine. termined as described Ref. 7 and references therein ; . The catalytic activity of each cytochrome P-450 isozyme was measured at 37 "C reconstituted system containing saturating amounts of NADPHcytochrome P-450 reductase and optimal amounts of dilauroylphosphatidylcholine, as described 2-7 ; . Each cytochrome P-450 isozyme.

Peter Wagner became a member of the Trenton Museum Society Board of Trustees because of his interest in horticulture. That may seem like an indirect path, but gardening is just one of many potential activities for TMS board members. Peter continues to garden at the Ellarslie mansion as part of the Gardening committee, but he's also chairman of the Development, Membership and Volunteer committee. Peter's background in gardening includes his job as a Horticulture Coordinator for a company based in Lansing, MI that produces plastic tags that label plants. When he moved to Trenton in 2001, Peter became the company's first telecommuter. Because of flexibility in his schedule, Peter has also started a gardening business. Given this experience, it's not surprising that another Museum Society board member suggested Peter might enjoy working on the board's Gardening committee. Peter still works in the museum's garden, but as chairman of the Development, Membership and Volunteer committee, he keeps busy managing three important elements of the Trenton Museum Society. He is working to further develop the grant-writing capability of the committee to enable the Museum Society to obtain more and larger grants which will allow for development of its programs. Through the membership aspect of his committee, Peter works to and doxorubicin.