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RESULTS Validity of the j-Amylase Assay The modified assay of f-amylase see "Materials and Methods" ; , which is cheaper and more suitable for a large number of samples than the original one 19 ; , gave values which were directly proportional to the time of incubation and to the amount of extract. However, when the values of absorbance were above 0.6, the reaction slowed down data not shown ; . Because the test was originally developed for human aamylase, interference by barley a-amylase was tested. A sample 0.5 , g, 2 units, see Ref. 24 ; of purified a-amylase from barley gave an absorbance of 0.4 in the Testomar-assay. This indicates that the test is not fully specific for , 3-amylase. To test the effect of a-amylase in the actual assay conditions, an endosperm extract was made using grains germinated for 4 d, at which time the activities of a- and , 3-amylases are virtually maximal. When , 3-amylase in the extract was inactivated by treatment at 70C, the activity in the Testomarassay decreased to 0.5% of the activity before the treatment Table I ; . Because a-amylase had remained fully active, the interference caused by it can be at the highest 0.5%. On the other hand, a-amylase was completely inactivated by a treatment with EDTA, but this treatment had virtually no effect on the 3-amylase assay. These results show that high amounts of barley a-amylase give some activity in the test, but in the conditions used to assay , B-amylase in extracts of germinating barley, the interference by a-amylase is less than 0.5% of the activity of ti-amylase. The assay can therefore be used to measure specifically 6-amylase activity even in extracts of germinating barley grains, as already suggested by Mathewson and Seabourn 19 ; . This conclusion has been further supported by immunoprecipitating the , 3-amylase with a specific antiserum 15 ; . Immunoprecipitation removed 98% of the activity data not shown.
TABLE 5 REGRESSIONS TO EXPLAIN LOG LONG-TERM UNEMPLOYMENT PERCENTAGE RATE 19 OECD COUNTRIES, 198394 Equationa Endogenous Variableb 1 ; OLS LUNEMP 2 ; OLS TAX 29.634 4.57 * ; 0.007 ; -0.055 -0.053 ; 0.211 1.25 ; -0.046 -0.468 ; 3.092 1.19 ; 0.671 0.24 ; 2.699 3.48 * ; 3 ; OLS 4 ; TSLS.
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Clin Chem 1981; 27: 2002-7. Valenzuela GJ, Munson LA, Tarbaux NM, Parley JR. Timedependent changes in bone, placental, intestinal, and hepatic silkflhine phosphatase activities in serum during human pregnancy. Clin Chem 1987; 33: 1801-6. Schoenau B, Herzog KH, Boehles HJ. Liquid-chromatographic determination of isoenzymes of silknline phosphatase in serum and tissue homogenates. Clin Chem 1986; 32: 816-8. Manusaon P, Lofman 0, Larsson L. Determination of alkaline phosphatase isoenzymes in serum by HPLC with postcolumn reaction detection. J Chromatogr Biomed Appl ; 1992; 576: 79-86. Lawson GM, Katzmann JA, Kimlinger TK, O'Brien JF. Lolation and preliminary characterization of a monoclonal antibody that interacts preferentially with the liver iaoenzyme of human RlkRline phosphatase. Cliii Chem 1985; 31: 381-5. Duda R, O'Brien J Jr, Katzmann J, Peterson J, Mann K, Riggs B. Concurrent assays of circulating bone gla-protein and bone Alkgline phosphatase: effects of sex, age, and metabolic bone disease. J Clin Endocrunol Metab 1988; 66: 951-7. Hirano K, Matsumoto H, Tanaka T, HayashiY, lino 5, Duman V, Stigbrand T. Specific assays for human silkRline phosphatase isoenzymes. Chin Chim Acta 1987; 166: 265-73. Hill CS, Wolfert RL. The preparation of monoclonal antibodies which react preferentially with human bone ALP and not liver.
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MAGNETIC RESONANCE IMAGING OF CANINE LUMBOSACRAL DISEASE: A RETROSPECTIVE STUDY IN 181 DOGS. Corzo-Menendez N., Davies J.V. Davies White, Veterinary Specialists. Hertfordshire, England. Introduction: Diagnosis of canine lumbosacral disease requires demonstration of compression of the cauda equina or LS nerve roots. Survey and contrast radiography have been the conventional methods for diagnosis. Myelography will only provide information about the lumbosacral area if the thecal sac extends to this region. Epidurography can provide additional information; however, false positive and false negative results can be obtained. Advanced tomographic imaging CT or MRI ; is the most sensitive technique for diagnosis of lumbosacral disease. Purpose: This study was performed to assess the different breeds and age groups affected by canine lumbosacral disease, and then to correlate the MRI findings with the long-term surgical outcome. Material and Methods: One hundred and eighty one dogs with clinical signs and MRI findings consistent with lumbosacral disease cauda equina compression have been reviewed 1997-2002 ; . Clinical signs included; back pain, exercise intolerance, reluctance to jump and corresponding neurological deficits. The dogs were anaesthetised for the procedure and positioned in dorsal recumbency. The lumbosacral area was imaged using a 0.5T ultra lightweight, active shielded superconducting magnet Philips Gyroscan T5-II ; . The sequences collected using a Quadrature T Lspinal coil were, T2W sagittal, T1W sagittal and T1W transverse through the lumbosacral disc space. Breed groups were assigned to three categories: giant- 50kg, large- 25-50kg, medium small 25kg. Age groups were assigned to four categories: 1-3 yo, 4-6 yo, 7-9 yo, 10-12 yo with a range of 1 to years old. Results: Demographics 181 dogs ; : Breeds: giant- 6%, large- 61.8%, medium small- 32%, Sex: female42.8%, male- 57.1%, Age: 1-3 yo- 27.6%, 4-6 yo- 28.5%, 7-9 yo- 29.4%, 10-12 yo- 14.2% MRI findings: The images were viewed for possible loss of epidural fat signal, intervertebral disc dehydration, intervertebral disc protrusion into the vertebral canal or foramen, and nerve root impingement by osteophyte formation. Surgical outcome: 30% of the 181 cases were referred for MR imaging alone, 40% were considered non-surgical candidates, and the reminder were subjected to dorsal laminectomy and discectomy. The results of surgery were assessed by owner questionnaire. Discussion Conclusion: This study has revealed that lumbosacral degenerative disc disease should be considered in a wider age group and different breed prevalence than previously described De Risio and others, 2000 ; . It is proposed that the success of surgery reflects the improved diagnostic capability of MR imaging in this particular condition, when compared with other diagnostic techniques plain radiography, myelography, epidurography, etc. ; . References: De Risio L., Thomas W. B., Sharp N. J. Degenerative lumbosacral stenosis. Veterinary Clinics of North America: Small Animal Practice, 2000, 30 1 ; : 111-32, vi. Davies J. V. MRI of the cauda equina. 9th Annual Congress of the European Society of Veterinary Orthopaedics and Traumatology. April 16th 19th 1998, Munich, Germany. Ramirez III O., Thrall D.E. A review of imaging techniques for canine cauda equina syndrome. Veterinary Radiology and Ultrasound, 1998, 39 4 ; : 283-296.
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